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Necessary cookies are absolutely essential for the website to function properly. This category only includes cookies that ensures basic functionalities and security features of the website. These cookies do not store any personal information.Phospholipase activity in the plasma of colorectal cancer patients.
An increase in the phospholipase activity in the plasma of colorectal cancer patients has been observed previously. The present study investigates if phospholipase A2 and C activities in the plasma are influenced by the underlying malignant disease. Enzyme activities were determined in 104 samples of the patients’ plasma and compared to those in 62 samples of the cancer-free controls. The plasma of colorectal cancer patients had increased phospholipase A2 and C activities compared to the controls. Phospholipase A2 activity was determined by releasing free fatty acids from dipalmitoylphosphatidylcholine/egg yolk lecithin or dipalmitoylphosphatidylcholine/triolein and phospholipase C activity by catalyzing the hydrolysis of a diacyl phospholipid phosphatidylcholine/phosphatidylinositol in a detergent extraction assay. Phospholipase A2 and C activities in the plasma were also determined by the use of a chromogenic water-soluble substrate. The differences in phospholipase activities were highly significant for both substrates used. The detection of increased phospholipase activities in the plasma of colorectal cancer patients suggests that phospholipids

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